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Microfluidic spore isolation and single-cell viability assessment platforms

Agricultural Microbiology

Spore-Forming Bacteria Enumeration and Viability Testing

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Dormancy mechanisms and metabolic state detection in bacterial spores Advanced flow cytometry protocols for rapid spore enumeration Germination kinetics and spore coat permeability under stress conditions qPCR and ddPCR assays for species-specific spore quantification Metabolic profiling and viability prediction via Raman spectroscopy High-throughput image analysis for morphological viability assessment Exosporium composition and outer membrane integrity biomarkers Enzymatic activity assays for spore metabolic competence evaluation Comparative genomics of spore-forming taxa and viability gene expression

Agricultural Microbiology › Spore-Forming Bacteria Enumeration and Viability Testing

Microfluidic spore isolation and single-cell viability assessment platforms

This study develops integrated microfluidic devices for rapid spore isolation, segregation, and real-time viability monitoring at single-cell resolution. The platform technology enables unprecedented mechanistic understanding of heterogeneity in spore population responses to germination signals.

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