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PAM-Adjacent Sequence Effects on Specificity

Molecular Eugenics

CRISPR Off-Target Effects Molecular Analysis

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PAM-Adjacent Sequence Effects on Specificity

Interns will conduct systematic studies on how nucleotide sequences flanking the PAM (Protospacer Adjacent Motif) region affect off-target recognition and cleavage efficiency. Research will involve mutagenesis studies, kinetic analysis, and development of PAM variant libraries for improved targeting specificity.

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