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CRISPR-Cas9 Targeted Mutagenesis of R-Gene Allelic Variants

Agriculture Plant Pathology

CRISPR Resistance Gene Engineering in Crops

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Multiplexed CRISPR Base Editing for Pyramiding Stacked Resistance Traits CRISPR-Mediated Epigenetic Silencing of Disease Susceptibility Factor Genes Prime Editing for Precise R-Gene Functional Domain Engineering CRISPR Off-Target Effects Assessment in Polyploid Crop Genomes CRISPR-Enabled Functional Validation of Candidate R-Genes from Wild Germplasm Inducible CRISPR-dCas9 Transcriptional Activation of Silent Resistance Loci CRISPR-Mediated Rational Design of Broad-Spectrum Pan-Pathotype R-Genes CRISPR-Derived Molecular Breeding Markers for Resistance Gene Stacking Optimization Phytochrome-Linked CRISPR Activation of Light-Responsive Immune Signaling Pathways

Agriculture Plant Pathology › CRISPR Resistance Gene Engineering in Crops

CRISPR-Cas9 Targeted Mutagenesis of R-Gene Allelic Variants

This research investigates the precise editing of resistance gene alleles using CRISPR-Cas9 to generate novel functional variants with enhanced pathogen recognition capabilities. The study yields comprehensive understanding of how allelic diversity at R-loci can be engineered to broaden spectrum resistance against evolving pathogen populations.

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