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Multiplexed CRISPR Base Editing for Pyramiding Stacked Resistance Traits

Agriculture Plant Pathology

CRISPR Resistance Gene Engineering in Crops

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CRISPR-Cas9 Targeted Mutagenesis of R-Gene Allelic Variants CRISPR-Mediated Epigenetic Silencing of Disease Susceptibility Factor Genes Prime Editing for Precise R-Gene Functional Domain Engineering CRISPR Off-Target Effects Assessment in Polyploid Crop Genomes CRISPR-Enabled Functional Validation of Candidate R-Genes from Wild Germplasm Inducible CRISPR-dCas9 Transcriptional Activation of Silent Resistance Loci CRISPR-Mediated Rational Design of Broad-Spectrum Pan-Pathotype R-Genes CRISPR-Derived Molecular Breeding Markers for Resistance Gene Stacking Optimization Phytochrome-Linked CRISPR Activation of Light-Responsive Immune Signaling Pathways

Agriculture Plant Pathology › CRISPR Resistance Gene Engineering in Crops

Multiplexed CRISPR Base Editing for Pyramiding Stacked Resistance Traits

This investigation explores simultaneous engineering of multiple R-genes through base editing technology to create polygenic resistance without breeding introgression. The research demonstrates accelerated pyramiding strategies that produce durable, multi-layered defense mechanisms with reduced linkage drag and shortened breeding timelines.

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