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CRISPR-Mediated Rational Design of Broad-Spectrum Pan-Pathotype R-Genes

Agriculture Plant Pathology

CRISPR Resistance Gene Engineering in Crops

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CRISPR-Cas9 Targeted Mutagenesis of R-Gene Allelic Variants Multiplexed CRISPR Base Editing for Pyramiding Stacked Resistance Traits CRISPR-Mediated Epigenetic Silencing of Disease Susceptibility Factor Genes Prime Editing for Precise R-Gene Functional Domain Engineering CRISPR Off-Target Effects Assessment in Polyploid Crop Genomes CRISPR-Enabled Functional Validation of Candidate R-Genes from Wild Germplasm Inducible CRISPR-dCas9 Transcriptional Activation of Silent Resistance Loci CRISPR-Derived Molecular Breeding Markers for Resistance Gene Stacking Optimization Phytochrome-Linked CRISPR Activation of Light-Responsive Immune Signaling Pathways

Agriculture Plant Pathology › CRISPR Resistance Gene Engineering in Crops

CRISPR-Mediated Rational Design of Broad-Spectrum Pan-Pathotype R-Genes

This research uses structural biology and CRISPR-based directed evolution to engineer chimeric R-proteins recognizing multiple pathotype-specific effector variants simultaneously. The investigation produces durable, rationally designed resistance genes with expanded recognition repertoires reducing pathogen virulence evolution pressures.

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